Author Correction: A single-cell and single-nucleus RNA-Seq toolbox for fresh and frozen human tumors

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Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq
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Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq

Published on: August 25, 2020

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Michal Slyper1, Caroline B M Porter1, Orr Ashenberg1

  • 1Klarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA, USA.

Nature Medicine
|June 27, 2020
PubMed

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RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...