BIOANALYSIS AND BIOTRANSFORMATION OF OLIGONUCLEOTIDE THERAPEUTICS BY LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY

Area of Science:

• Biochemistry
• Analytical Chemistry
• Pharmacology

Background:

• Eight new oligonucleotide therapies approved since 2016 have increased interest in their analysis.
• Oligonucleotide-based therapeutics are gaining prominence in various medical fields.
• Understanding the metabolism and biotransformation of these drugs is crucial for their effective use.

Purpose of the Study:

• To provide a comprehensive overview of oligonucleotide analysis.
• To review current analytical methodologies for studying oligonucleotide metabolism.
• To identify challenges and future perspectives in the field of oligonucleotide bioanalysis.

Main Methods:

• Review of existing literature on oligonucleotide therapies and bioanalytical techniques.
• Discussion of sample preparation methods relevant to oligonucleotide analysis.
• Overview of liquid chromatography-mass spectrometry (LC-MS) applications in oligonucleotide quantification.

Main Results:

• Current analytical methods, including sample preparation and LC-MS, are discussed.
• The review highlights the state-of-the-art in detecting and quantifying oligonucleotides.
• Limitations in current detection and quantitation methods are identified.

Conclusions:

• There is a significant need for enhanced bioanalytical tools for oligonucleotide analysis.
• Addressing the challenges in oligonucleotide bioanalysis is key to advancing therapeutic applications.
• Future research should focus on developing more sensitive and robust analytical methods.