Related Experiment Video
Updated: Dec 17, 2025
CD Spectroscopy to Study DNA-Protein Interactions
Published on: February 10, 2022
Multispectroscopic analysis, atomic force microscopy, molecular docking and molecular dynamic simulation studies of
Nahid Shahabadi1,2, Mohammad Mahdavi1, Badri Z Momeni3
1Department of Inorganic Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Iran.
Abstract:
In this study, the spectroscopic methods (UV-vis, fluorimetric), Atomic force microscopy, and computational studies (molecular docking and molecular dynamic simulation) were used to investigate the interaction of [SnMe2Cl2(Me2phen)] complex with CT-DNA in the presence of glucose. The results showed the complex in the medium containing glucose has less effect on calf thymus DNA (ct-DNA) than the medium without glucose. Cytotoxicity of [SnMe2Cl2(Me2phen)] complex on MCF-7 cells was examined and showed Sn(IV) complex possesses potential cytotoxicity against this cell line. Molecular docking study showed that Sn(IV) complex interacts with DNA by groove binding mode. Radius of gyration (Rg) was smaller upon binding of the Sn(IV) complex suggesting a more compact structure of DNA in the presence of Sn(IV) complex.Communicated by Ramaswamy H. Sarma.
