Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Computed Tomography01:10

Computed Tomography

7.8K
Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
7.8K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.7K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.7K
Imaging Studies III: Computed Tomography01:27

Imaging Studies III: Computed Tomography

194
DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
194

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrating replication kinetics and ultrastructural analysis to identify targets for optimizing rVSV bioproduction.

Microbiology spectrum·2026
Same author

RapTB: a lung-derived hemoglobin fragment with activity against <i>Mycobacterium tuberculosis</i>.

Frontiers in microbiology·2025
Same author

Scanning Transmission Electron Microscopy Tomography in Virology: 3D Imaging of High-pressure Frozen, Freeze-substituted Samples.

Journal of visualized experiments : JoVE·2025
Same author

Biophysical characterization and solution structure of the cannulae-forming protein CanA from the hyperthermophilic archaeon Pyrodictium abyssi.

Scientific reports·2025
Same author

Provirus deletion from <i>Haloferax volcanii</i> affects motility, stress resistance, and CRISPR RNA expression.

microLife·2025
Same author

MCSP<sup>+</sup> metastasis founder cells activate immunosuppression early in human melanoma metastatic colonization.

Nature cancer·2025

Related Experiment Video

Updated: Dec 17, 2025

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
08:55

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

5.6K

Dual-axis STEM tomography at 200 kV: Setup, performance, limitations.

Reinhard Rachel1, Paul Walther2, Christine Maaßen3

  • 1Center for Electron Microscopy, University of Regensburg, Regensburg, Germany.

Journal of Structural Biology
|June 27, 2020
PubMed
Summary

Scanning transmission electron microscopy (STEM) tomography is now feasible on a 200 kV microscope, offering high-quality 3D cellular structure analysis. This advancement provides reliable data for understanding complex cell biology, even with thicker samples.

Keywords:
Dual-axis tomographyElectron tomographySTEM tomography

More Related Videos

Visualization of Organelles In Situ by Cryo-STEM Tomography
08:37

Visualization of Organelles In Situ by Cryo-STEM Tomography

Published on: June 23, 2023

2.9K
Using High Resolution Computed Tomography to Visualize the Three Dimensional Structure and Function of Plant Vasculature
11:49

Using High Resolution Computed Tomography to Visualize the Three Dimensional Structure and Function of Plant Vasculature

Published on: April 5, 2013

21.5K

Related Experiment Videos

Last Updated: Dec 17, 2025

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
08:55

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

5.6K
Visualization of Organelles In Situ by Cryo-STEM Tomography
08:37

Visualization of Organelles In Situ by Cryo-STEM Tomography

Published on: June 23, 2023

2.9K
Using High Resolution Computed Tomography to Visualize the Three Dimensional Structure and Function of Plant Vasculature
11:49

Using High Resolution Computed Tomography to Visualize the Three Dimensional Structure and Function of Plant Vasculature

Published on: April 5, 2013

21.5K

Area of Science:

  • Cell Biology
  • Microscopy
  • Structural Biology

Background:

  • Understanding cellular structures is key to interpreting biological processes.
  • Electron tomography (ET) has significantly advanced 3D organelle analysis.
  • Previous ET studies primarily utilized 300 kV transmission electron microscopes.

Purpose of the Study:

  • To implement and evaluate scanning transmission electron microscopy (STEM) tomography on a 200 kV field emission gun (FEG) transmission electron microscope.
  • To assess the feasibility, reliability, and data quality of this approach for 3D structural analysis.

Main Methods:

  • Implementation of STEM tomography on a 200 kV FEG-TEM.
  • Tuning the condenser for a small illumination aperture beam.
  • Dual-axis data acquisition and analysis of sample thickness and data quality.

Main Results:

  • Successful implementation of STEM tomography on the 200 kV FEG-TEM.
  • Demonstrated high reliability and excellent data quality.
  • Achieved high-quality 3D reconstructions from plastic sections up to 900 nm thick.

Conclusions:

  • STEM tomography is a viable and promising technique on 200 kV FEG-TEMs.
  • This method enhances the capability for detailed 3D analysis of cellular ultrastructures.
  • The approach offers a reliable alternative for high-resolution structural investigations in cell biology.