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Molecular identification of Cryptococcus gattii from cerebrospinal fluid using single-cell sequencing: A case study
Min Chen1, Nan Hong2, Shan Hu3
1Beijing Key Laboratory of New Molecular Diagnosis Technologies for Infectious Disease, Department of Biotechnology, Beijing Institute of Radiation Medicine, Beijing, China; Shanghai Key Laboratory of Molecular Medical Mycology, Department of Dermatology, Changzheng Hospital, Second Military Medical University, Shanghai, China.
Single-cell sequencing identified Cryptococcus gattii in a patient with cryptococcal meningitis when standard tests failed. This novel approach offers a powerful tool for diagnosing difficult-to-culture fungal infections.
Area of Science:
- Medical Mycology
- Infectious Diseases
- Genomics
Background:
- Cryptococcal meningitis (CM) can present with atypical symptoms, complicating diagnosis.
- Conventional methods for identifying fungal pathogens in cerebrospinal fluid (CSF) can be challenging due to difficulties in cultivation.
