An accurate and universal approach for short-exposure-time microscopy image enhancement

Fluorescence microscopy imaging has become an essential technique in the biology and biomedical science which can provide comprehensive visualization of many biological processes, and the exposure time is one of the most critical parameters for fluorescence microscopy imaging. Short-exposure-time (SET) imaging overcomes the limitations of photo-bleaching and photo-toxicity, allowing comprehensive visualization of the biological processes. Unfortunately, SET images deteriorate the signal to noise ratio and the image quality when compared with the long-exposure-time (LET) images. Therefore, we introduce a data-driven microscopy image enhancement network (MIEN) to improve the quality of SET microscopy images without requiring any manual intervention, facilitating the production of high-resolution and high contrast images. The universal property and accuracy of the proposed network are validated on 38,500 real fluorescence microscopy images, which contain different object contents and are collected from various exposure time ratios and fluorescence microscopes platforms. Experimental results demonstrate that the proposed MIEN model is effective to enhance the quality of SET fluorescence microscopy images, and can be used to observe delicate changes in cells, tissues and organs with low photo-bleaching and photo-toxicity.