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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

Updated: Dec 17, 2025

Test Samples for Optimizing STORM Super-Resolution Microscopy

Test Samples for Optimizing STORM Super-Resolution Microscopy

Published on: September 6, 2013

Publisher Correction: DeepSTORM3D: dense 3D localization microscopy and PSF design by deep learning.

Elias Nehme^1,2, Daniel Freedman³, Racheli Gordon²

¹Department of Electrical Engineering, Technion, Haifa, Israel.

|June 28, 2020

Summary

This study has been amended. Please refer to the updated version for the latest findings and analysis.

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Area of Science:

Not specified in the abstract.

Context:

The original paper has been updated.
An amendment is available.

Purpose:

To inform readers about the published amendment.
To direct readers to the updated paper.

Summary:

An amendment to this research paper has been published.
The amendment can be accessed via a link at the top of the original paper.

Impact:

Ensures readers access the most current and accurate information.
Facilitates informed understanding based on revised content.