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Related Experiment Video

Updated: Dec 17, 2025

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
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Microfluidic Paper-Based Analytical Device for Histidine Determination.

Akimitsu Kugimiya1, Akane Fujikawa2, Xiao Jiang3

  • 1Department of Biomedical Information Sciences, Graduate School of Information Sciences, Hiroshima City University, 3-4-1 Ozuka-higashi, Asaminami-ku, Hiroshima, 731-3194, Japan. kugimiya@hiroshima-cu.ac.jp.

Applied Biochemistry and Biotechnology
|June 28, 2020
PubMed
Summary
This summary is machine-generated.

A new paper-based device detects histidine using an enzyme and a color change. This simple, cost-effective method allows selective histidine quantification in a specific concentration range.

Keywords:
Amino acidAminoacyl-tRNA synthetaseBiosensingLaminated paper-based analytical deviceMicrofluidics

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Materials Science

Background:

  • Histidine is an essential amino acid with critical roles in biological systems.
  • Accurate and accessible detection methods for histidine are valuable in various scientific fields.
  • Existing detection methods may be complex or costly.

Purpose of the Study:

  • To develop a simple, cost-effective, and selective paper-based analytical device for histidine detection.
  • To utilize histidyl-tRNA synthetase (HisRS) for specific histidine recognition.
  • To establish a colorimetric detection method based on the molybdenum blue reaction.

Main Methods:

  • Fabrication of a laminated paper-based analytical device (LPAD) using chromatography filtration paper and laminate films.
  • Employing histidyl-tRNA synthetase (HisRS) for selective histidine binding.
  • Colorimetric detection of histidine via the molybdenum blue reaction.
  • Optimization of analytical conditions and determination of the detectable concentration range.

Main Results:

  • The developed LPAD demonstrated selective quantification of histidine.
  • The detectable concentration range for histidine was determined to be from 1 to 100 μM.
  • The fabrication process for the LPAD was found to be simple and cost-effective.
  • The device utilizes a colorimetric readout based on the molybdenum blue reaction.

Conclusions:

  • A novel, simple, and cost-effective laminated paper-based analytical device (LPAD) for histidine detection has been successfully developed.
  • The LPAD, utilizing HisRS and a molybdenum blue reaction, offers selective histidine quantification within a 1-100 μM range.
  • This paper-based sensor presents a promising tool for accessible histidine analysis in various applications.