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Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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Shortening the sgRNA-DNA interface enables SpCas9 and eSpCas9(1.1) to nick the target DNA strand

Rong Fan1,2,3, Zhuangzhuang Chai2,3, Sinian Xing2

  • 1Key Laboratory of Agricultural Water Resources, Hebei Laboratory of Agricultural Water-Saving, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, 050022, China.

Science China. Life Sciences
|June 28, 2020
PubMed

Keywords:
DSBSpCas9co-editingeSpCas9(1.1)nickasetruncated spacer

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