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Related Experiment Video

Updated: Dec 17, 2025

Fabrication of Biologically Derived Injectable Materials for Myocardial Tissue Engineering
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Mitral Valve Posterior Leaflet Reconstruction Using Extracellular Matrix: In Vitro Evaluation.

Marcell J Tjørnild1,2,3, Søren W Sørensen4,5, Lisa Carlson Hanse4,5

  • 1Department of Cardiothoracic and Vascular Surgery, Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, 8200, Aarhus, Denmark. marcell@clin.au.dk.

Cardiovascular Engineering and Technology
|June 28, 2020
PubMed
Summary

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Functional competency of a novel 2-ply vacuum-pressed biological scaffold for entire posterior mitral valve reconstruction.

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This summary is machine-generated.

This study shows a new surgical technique using small intestinal submucosa extracellular matrix for mitral valve reconstruction effectively restored valve function in vitro. The reconstructed mitral valves demonstrated comparable anatomy and function to native valves.

Area of Science:

  • Biomedical Engineering
  • Cardiovascular Surgery
  • Regenerative Medicine

Background:

  • Mitral valve disease affects millions globally, necessitating advanced reconstructive techniques.
  • Current surgical options for posterior mitral leaflet repair have limitations.
  • Extracellular matrix biomaterials offer potential for tissue regeneration in cardiac repair.

Purpose of the Study:

  • To evaluate the anatomical and functional outcomes of a novel in vitro surgical reconstruction of the posterior mitral leaflet and chordae tendineae.
  • To assess the efficacy of a 2-ply small intestinal submucosa extracellular matrix patch for mitral valve repair.

Main Methods:

  • Utilized seven porcine mitral valves in a left heart simulator, serving as their own controls.
  • Excised and reconstructed the posterior mitral leaflet and chordae tendineae using a small intestinal submucosa extracellular matrix patch.
Keywords:
2-ply small intestinal submucosa extracellular matrixCorMatrixMitral valve reconstructionMitral valve repairPapillary muscle forcePosterior leaflet reconstruction

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  • Characterized reconstruction via geometric analysis, papillary muscle force, annular tethering force, and leaflet pressure force.

    • Main Results:

    • Reconstructed valves exhibited full functionality without regurgitation, tearing, or rupture up to 120 mmHg.
    • Leaflet areas and coaptation line configuration were preserved, though the coaptation midpoint shifted posteriorly.
    • Anterior papillary muscle force increased significantly post-reconstruction, while other forces remained unchanged.

    Conclusions:

    • The novel surgical technique using small intestinal submucosa extracellular matrix provides comparable mitral valve anatomy and function to native valves in an in vitro model.
    • These findings suggest the potential of this biomaterial for posterior mitral leaflet reconstruction.
    • Further in vivo studies are warranted to validate these promising results.