Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Transcytosis of IgG

Transcytosis of IgG

Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...

Embryonic Connective Tissues

Embryonic Connective Tissues

During early development, the embryo forms two types of connective tissues— the mesenchyme and mucoid connective tissue.
The mesenchyme is the first connective tissue that emerges in the developing embryo. It consists of loosely arranged multipotent mesenchymal cells and reticular fibers in the extracellular matrix. This loose arrangement allows easy migration of cells, which is essential for germ layer positioning, patterning, and organ morphogenesis during embryonic development.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Placental Tissues as Biomaterials in Regenerative Medicine.

BioMed research international·2022

See all related articles

Search research articles

Related Experiment Video

Updated: Dec 17, 2025

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

Published on: November 28, 2019

Intermediate layer contribution in placental membrane allografts.

Annelise Roy¹, Sarah Griffiths¹

¹Research and Development Department, StimLabs, LLC, Roswell, GA, USA.

Journal of Tissue Engineering and Regenerative Medicine

|June 28, 2020

Summary

Dehydrated complete human placental membrane (dCHPM) allografts retain structural and regulatory proteins. The intermediate layer (IL) is rich in signaling components crucial for wound healing and tissue repair.

Keywords:

allograft amnion chorion extracellular matrix placental membrane wound healing

More Related Videos

Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue

Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue

Published on: June 6, 2016

Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae

Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae

Published on: April 30, 2018

Related Experiment Videos

Last Updated: Dec 17, 2025

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

Published on: November 28, 2019

Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue

Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue

Published on: June 6, 2016

Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae

Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae

Published on: April 30, 2018

Area of Science:

Regenerative Medicine
Biomaterials Science
Wound Healing Research

Background:

Placental membrane (PM) allografts are utilized for chronic wound treatment.
Native PM consists of amnion, chorion, and an intermediate layer (IL) rich in healing components.
Previous allografts often excluded the IL, potentially limiting therapeutic benefits.

Purpose of the Study:

To characterize the dehydrated complete human placental membrane (dCHPM) allograft.
To evaluate the structural and proteomic contribution of the IL in dCHPM allografts.
To compare dCHPM allografts with native PM to assess retained healing factors.

Main Methods:

Histological and proteomic analyses were performed on native PM and dCHPM allografts.
The structural and proteomic composition of the IL was specifically investigated.
dCHPM processing using the Clearify™ method was detailed.

Main Results:

dCHPM allografts retain the majority of matrix structures and regulatory proteins from native PM.
The IL contains over 900 regulatory and signaling proteins, including growth factors and interleukins.
The IL's proteomic profile is consistent with the entire dCHPM allograft, highlighting its importance.

Conclusions:

The IL is a significant source of regulatory proteins essential for wound healing processes.
dCHPM allografts preserve key structural and regulatory components of the native PM.
The intact PM, including the IL, may enhance barrier and healing properties of placental membrane allografts.