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The Effect of Aging on Tissues

The Effect of Aging on Tissues

Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...

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Related Experiment Video

Updated: Dec 17, 2025

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Published on: May 18, 2017

Global transcriptomic changes occur in aged mouse podocytes.

Yuliang Wang¹, Diana G Eng², Natalya V Kaverina²

¹Paul G. Allen School of Computer Science and Engineering, University of Washington, Seattle, Washington, USA; Institute for Stem Cell & Regenerative Medicine, University of Washington, Seattle, Washington, USA.

Kidney International

|June 28, 2020

Summary

This summary is machine-generated.

Aging podocytes exhibit distinct transcriptional changes, including altered immune responses and suppressed metabolic processes, impacting kidney health. Understanding these molecular shifts aids in developing targeted interventions for aging kidneys.

Keywords:

RNA sequencing aging differentially expressed genes gene ontology glomerulus podocyte

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Author Spotlight: Generation of Patient-Derived Podocytes from Skin Biopsies

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Last Updated: Dec 17, 2025

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Published on: May 26, 2023

Area of Science:

Nephrology
Gerontology
Molecular Biology

Background:

Aging kidneys are more susceptible to glomerular diseases due to structural and functional podocyte changes.
Advanced age in mice (22-24 months) mirrors human aging (70+ years), making them a relevant model for studying kidney aging.

Purpose of the Study:

To identify and characterize the transcriptional alterations in glomerular podocytes during advanced aging.
To understand the molecular mechanisms underlying age-related podocyte dysfunction and kidney susceptibility.

Main Methods:

RNA sequencing (RNA-seq) was performed on isolated podocytes from young and aged mice.
Reporter-labeled (tdTomato) podocytes were used for precise isolation and analysis.
Bioinformatic analyses including pathway enrichment and transcription factor identification were conducted.

Main Results:

2,494 differentially expressed genes were identified in aged podocytes compared to young ones.
Upregulated pathways in aged podocytes included immune responses, non-coding RNA metabolism, and MAP kinase signaling.
Downregulated pathways involved developmental, morphogenesis, and metabolic processes; canonical podocyte markers decreased, while apoptotic and senescence genes increased.

Conclusions:

Aging induces a distinct transcriptional signature in podocytes, characterized by immune activation and metabolic decline.
These changes contribute to podocyte loss and increased kidney vulnerability with age.
The findings offer insights for biomarker discovery and therapeutic targeting to promote healthy kidney aging.