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CRABP2 and FABP5 expression levels in diseased and normal pancreas

Christine S Hughes1, Jo-Anne ChinAleong2, Hemant M Kocher3

  • 1Centre for Tumour Biology, Barts Cancer Institute - a CR-UK Centre of Excellence, Queen Mary University of London, Charterhouse Square, EC1M 6BQ London, UK.

Annals of Diagnostic Pathology
|June 29, 2020
PubMed

Insights

Cellular retinoic acid binding protein 2 (CRABP2) and fatty acid binding protein 5 (FABP5) levels differ in pancreatic tissues. These protein ratios may serve as biomarkers for pancreatic ductal adenocarcinoma (PDAC) progression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Stromal targeting with All trans retinoic acid (ATRA) shows promise for pancreatic ductal adenocarcinoma (PDAC) treatment.
  • Intracellular ATRA transport to nuclear receptors is mediated by fatty acid binding protein 5 (FABP5) or cellular retinoic acid binding protein 2 (CRABP2), influencing gene transcription and cell phenotype.

Purpose of the Study:

  • To investigate the expression levels and ratios of FABP5 and CRABP2 in pancreatic tissues from patients with PDAC, chronic pancreatitis (CP), and cholangiocarcinoma (CC).
  • To explore the potential of FABP5 and CRABP2 as biomarkers for pancreatic diseases and tumor progression.

Main Methods:

  • Analysis of FABP5 and CRABP2 expression in pancreatic tissues (PDAC, CP, CC) and normal tissues.
  • Comparison of protein expression and ratios between activated/quiescent fibroblasts, pancreatic stellate cells (PSCs), and epithelial cells.
  • Correlation analysis of protein levels and ratios with disease state and potential clinical outcomes.

Main Results:

  • Lower CRABP2 and FABP5 expression observed in activated PSCs from PDAC, CC, and CP tissues compared to quiescent fibroblasts.
  • Quiescent fibroblasts exhibited a high FABP5:CRABP2 ratio; PSCs in non-PDAC diseases showed a low ratio.
  • PDAC PSCs displayed variable FABP5:CRABP2 ratios. CRABP2 expression was lower in cancerous epithelial cells (PDAC) versus normal cells, a trend also seen in CP and CC.
  • PDAC epithelial cells showed similar FABP5 expression to normal cells, but normal cells had a higher FABP5:CRABP2 ratio.

Conclusions:

  • FABP5 and CRABP2 expression patterns and their ratios differ significantly between normal and diseased pancreatic tissues, including PDAC.
  • These distinct protein expression profiles and ratios in fibroblasts and epithelial cells suggest their potential as valuable biomarkers for pancreatic diseases and tumor progression.
  • Further in vitro studies are warranted to confirm these findings and validate FABP5 and CRABP2 as reliable biomarkers.
Keywords:
AtraCancerImmunoflourescencePancreasRetinoids

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