Colorimetric detection of insulin in human serum using GO/AuNPs/TX-100 nanocomposite

In this study, graphene oxide/gold nanoparticles/Triton X-100 nanocomposites (GO/AuNPs/TX-100) were synthesized using the sonochemical method and their ability in ultrasound-assisted colorimetric detection of insulin was investigated. The synthesized GO/AuNPs/TX-100 nanocomposites were characterized by UV-visible absorption spectroscopy and TEM analysis. The interaction between the nanocomposites and insulin was observed by both naked eye and optical absorption spectroscopy. The GO/AuNPs/TX-100 nanocomposites displayed apparent color changes (red to blue) and absorption spectra changes (decreasing of the band around 528 nm and appearance of a new red-shifted band at 640 nm) in presence of insulin. The interaction mechanism of the nanocomposites and insulin was discussed. It is based on the special structure of insulin, that insulin can be easily self-assemble into the GO/AuNP/TX-100 nanocomposites and can also play the role of a bridge between two different GO/AuNPs/TX-100 nanocomposites by peptide chains. The effective parameters for insulin detection were optimized. The colorimetric method was used for quantification of insulin in the range of 2-300 ng mL^-1 with a detection limit of 0.1 ng mL^-1. Moreover, the relative standard deviation of the method was 3.1 and 2.7% (n = 10) at concentrations of 50 and 200 ng mL^-1, respectively on the same day and 4.8% at a concentration (200.0 ng mL^-1) on five consecutive days. The present method was utilized for insulin assay in human blood serums with satisfactory results.