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SiC-functionalized fluorescent aptasensor for determination of Proteus mirabilis
Wenyan Yao1, Jian Shi2, Jiang Ling1
1Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha, 410013, Hunan, China.
Mikrochimica Acta
|June 29, 2020
Summary
A novel fluorescent aptasensor using silicon carbide quantum dots (SiC QDs) can detect Proteus mirabilis bacteria rapidly. This method shows promise for identifying P. mirabilis in forensic food poisoning investigations.
Area of Science:
- Nanotechnology
- Biotechnology
- Analytical Chemistry
Background:
- Accurate and rapid detection of bacterial pathogens like Proteus mirabilis is crucial for public health and forensic investigations.
- Traditional methods for bacterial identification can be time-consuming and labor-intensive.
- Development of sensitive and specific biosensors is essential for timely pathogen detection.
Purpose of the Study:
- To develop and validate a novel fluorescent aptasensor for the sensitive and rapid determination of Proteus mirabilis.
- To utilize silicon carbide quantum dots (SiC QDs) modified with aptamers for enhanced bacterial recognition.
- To assess the aptasensor's performance in various sample matrices, including food and simulated forensic samples.
Main Methods:
- Synthesis of SiC quantum dots (SiC QDs) via a one-pot hydrothermal method.
- Conjugation of amino-modified aptamers specific to P. mirabilis onto the surface of SiC QDs.
- Utilizing the fluorescence quenching mechanism of DNA-SiC QDs upon binding with P. mirabilis for detection.
- Characterization of the aptasensor's performance, including linear range and limit of detection.
Main Results:
- The synthesized SiC QDs had particle sizes of approximately 14 nm.
- The aptasensor demonstrated rapid detection of P. mirabilis within 35 minutes.
- A linear detection range from 10^3 to 10^8 CFU mL^-1 with a limit of detection of 526 CFU mL^-1 was achieved.
- The aptasensor showed good accuracy (87.6-104.5%) and recovery rates (85-110.2%) in pure milk samples.
- Satisfactory coincidence rates were obtained when testing simulated forensic samples (milk, milk powder, blood, urine) compared to standard bacterial isolation methods.
Conclusions:
- The developed aptamer-modified SiC QD fluorescent aptasensor is a sensitive and efficient tool for P. mirabilis detection.
- The aptasensor offers a rapid and reliable method for identifying P. mirabilis in complex matrices.
- This technology holds significant potential for applications in forensic food poisoning investigations and diagnostics.
