Related Experiment Video
Updated: Dec 17, 2025
An Ultrasonic Tool for Nerve Conduction Block in Diabetic Rat Models
Published on: October 20, 2017
Can troxerutin pretreatment prevent testicular complications in prepubertal diabetic male rats?
Afsaneh Ghadiri1, Fariba Mirzaei Bavil2,1, Gholam Reza Hamidian3
1Deptartment of Physiology, Tabriz University of Medical Sciences, Tabriz, Iran.
Objective:
The vast majority of type 1 diabetes leads to a higher prevalence of reproductive system's impairments. Troxerutin has attracted much attention owing to its favorable properties, including antihyperglycemic, anti-inflammatory, and antiapoptotic effects. This investigation was proposed to evaluate whether pretreatment with troxerutin could prevent apoptosis-induced testicular disorders in prepubertal diabetic rats.
Methods:
Fifty prepubertal male Wistar rats were randomly allocated into five groups: control (C), troxerutin (TX), diabetic (D), diabetic+troxerutin (DTX), and diabetic+insulin (DI). Diabetes was induced by 55 mg/kg of streptozotocin applied intraperitoneally. In TX and DTX groups, 150 mg/kg troxerutin was administered by oral gavage. Diabetic rats in DI group received 2-4 U NPH insulin subcutaneously. Troxerutin and insulin treatments were begun immediately on the day of diabetes confirmation. After 30 days, the testicular lipid peroxidation and antioxidant activity, apoptosis process, and stereology as well as serum glucose and insulin levels were assessed.
Results:
The results showed that diabetes caused a significant increase in the blood glucose, the number of TUNEL positive cells and tubules, and the malondialdehyde level as well as a significant decrease in serum insulin level compared to controls. The stereological analysis also revealed various alterations in diabetic rats compared to controls. Troxerutin treatment improved these alterations compared to the diabetic group.
Conclusion:
Troxerutin-pretreatment may play an essential role in the management of the type-1 diabetes-induced testicular disorders by decreasing blood glucose and modulating apoptosis.
Insights
Troxerutin pretreatment in diabetic rats reduced blood glucose and modulated apoptosis, preventing testicular disorders. This suggests troxerutin may be beneficial for managing type 1 diabetes complications.
Area of Science:
- Endocrinology
- Reproductive Biology
- Toxicology
Background:
- Type 1 diabetes frequently causes reproductive system impairments.
- Troxerutin exhibits antihyperglycemic, anti-inflammatory, and antiapoptotic properties.
- Apoptosis-induced testicular dysfunction is a concern in diabetic individuals.
Purpose of the Study:
- To investigate the protective effects of troxerutin pretreatment against testicular disorders in prepubertal diabetic rats.
- To evaluate troxerutin's impact on apoptosis and related markers in diabetic testes.
Main Methods:
- Fifty male Wistar rats were divided into control, troxerutin, diabetic, diabetic+troxerutin, and diabetic+insulin groups.
- Diabetes was induced using streptozotocin; troxerutin and insulin were administered orally and subcutaneously, respectively.
- After 30 days, testicular lipid peroxidation, antioxidant activity, apoptosis, stereology, and serum glucose/insulin levels were assessed.
Main Results:
- Diabetes significantly increased blood glucose, malondialdehyde, and TUNEL-positive cells while decreasing serum insulin.
- Stereological analysis showed significant testicular alterations in diabetic rats compared to controls.
- Troxerutin treatment notably improved these diabetes-induced alterations.
Conclusions:
- Troxerutin pretreatment demonstrated a protective effect against type 1 diabetes-induced testicular disorders in rats.
- Troxerutin's benefits appear linked to its ability to decrease blood glucose and modulate apoptosis.
- These findings suggest troxerutin's potential role in managing diabetes-related reproductive complications.
