Related Experiment Video
Updated: Dec 17, 2025
09:17
Optimizing Extracellular Vesicle Delivery Using a Core-Sheath 3D-Bioprinted Scaffold for Chronic Wound Management
Published on: February 28, 2025
691
Peptide Chitosan/Dextran Core/Shell Vascularized 3D Constructs for Wound Healing.
Paul R Turner1, Eoin Murray1, C John McAdam1
1Department of Chemistry, University of Otago, Dunedin 9054, New Zealand.
ACS Applied Materials & Interfaces
|June 30, 2020
Summary
This study introduces a novel core-shell 3D bioprinting method using biomimetic materials for regenerative medicine. The developed scaffolds promote cell viability and vascularization, accelerating wound healing.
Area of Science:
- Regenerative Medicine
- Biomaterials Science
- Tissue Engineering
Background:
- Vascularized networks are crucial for engineered tissues in regenerative medicine.
- Current strategies for creating vascularized scaffolds face challenges in mimicking natural tissue structures.
- 3D bioprinting offers a promising avenue for constructing complex, cell-laden tissue constructs.
Purpose of the Study:
- To develop a novel core-shell 3D bioprinting strategy for creating prevascularized scaffolds for wound care applications.
- To engineer cell-responsive bioinks using biomimetic materials for enhanced cell viability and tissue regeneration.
- To investigate the potential of these constructs in promoting vascularization and accelerating skin wound healing.
Main Methods:
- Utilized core-shell (c/s) extrusion 3D-bioprinting technology with a gelatin methacryloyl (GelMA) shell and a peptide-functionalized chitosan/dextran aldehyde (C/D) core.
- Developed custom bioinks incorporating human bone-marrow-derived mesenchymal stem cells (hBMSCs) and human umbilical vein endothelial cells (HUVECs).
- Employed GelMA's thermoreversible properties and UV photo-cross-linking for scaffold stabilization and mechanical integrity, alongside peptide functionalization (P15/MMP-2, P15/cRGD) for cell adhesion and growth.
Main Results:
- Successfully bioprinted organized microdesigns with excellent cell viability and subsequent vessel formation.
- Demonstrated natural, cord-like microvascularization with endothelial cell marker expression via immunofluorescence staining.
- Observed a twofold rate of wound closure in *in vitro* skin wound healing assays.
Conclusions:
- Core-shell 3D bioprinted peptide-CD/GelMA constructs provide a suitable microenvironment for stem and endothelial cell viability, delivery, and differentiation.
- This strategy represents a significant advancement towards engineering larger-scale regenerative, prevascularized tissues.
- The developed technology holds promise for novel cell-based therapies in wound care and regenerative medicine.
