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An innovative blood plasma separation method for a paper-based analytical device using chitosan functionalization.

Dami Kim1, SeJin Kim, Sanghyo Kim

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This study presents a novel microfluidic paper-based analytical device (μPAD) for efficient plasma separation and glucose measurement from whole blood. The device utilizes chitosan and wax barriers for cell isolation, enabling point-of-care diagnostics.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Materials Science

Background:

  • Point-of-care testing (POCT) requires efficient sample preparation.
  • Microfluidic paper-based analytical devices (μPADs) offer a low-cost platform for diagnostics.
  • Accurate glucose measurement is crucial for diabetes management.

Purpose of the Study:

  • To develop a novel μPAD for simultaneous plasma separation and glucose detection.
  • To functionalize a μPAD with chitosan for enhanced red blood cell isolation.
  • To validate the performance of the μPAD for quantitative glucose analysis.

Main Methods:

  • Fabrication of a two-dimensional μPAD using wax printing on chromatographic paper.
  • Functionalization of the plasma separation zone with chitosan.
  • Isolation of red blood cells using chitosan and wax barriers.
  • Colorimetric detection of glucose in separated plasma.
  • Digitization of colorimetric results using a scanner and calibration curve analysis.

Main Results:

  • Successful separation of plasma from whole blood without a membrane.
  • Effective isolation of red blood cells within the chitosan-functionalized zone.
  • Quantitative measurement of glucose concentration via colorimetric analysis.
  • Demonstration of the μPAD's utility for small-volume sample analysis.

Conclusions:

  • The developed μPAD provides an effective method for plasma separation and glucose measurement.
  • Chitosan functionalization enhances the performance of μPADs for blood sample processing.
  • This technology holds promise for developing advanced point-of-care diagnostic devices.