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Polytene Chromosomes

Polytene Chromosomes

Polytene chromosomes are giant interphase chromosomes with several DNA strands placed side by side. They were discovered in the year 1881 by Balbiani in salivary glands, intestine, muscles, malpighian tubules, and hypoderm of larvae Chironomus plumosus. Hence, these are also called "Salivary gland chromosomes." These are found in insects of the order Diptera and Collembola; in certain organs of mammals; and synergids, antipodes of flowering plants. Polytene chromosomes are also...

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Updated: Dec 17, 2025

A Drosophila Model to Study Wound-induced Polyploidization

A Drosophila Model to Study Wound-induced Polyploidization

Published on: June 9, 2020

A Drosophila Model to Study Wound-induced Polyploidization.

Erin C Bailey¹, Ari S Dehn¹, Kayla J Gjelsvik²

¹Biology Department, Boston College.

Journal of Visualized Experiments : Jove

|June 30, 2020

Summary

Wound-induced polyploidization (WIP) generates larger cells for tissue repair, offering advantages over cell proliferation. This study provides a method to investigate WIP in fruit flies, identifying key genes like Myc.

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Last Updated: Dec 17, 2025

A Drosophila Model to Study Wound-induced Polyploidization

A Drosophila Model to Study Wound-induced Polyploidization

Published on: June 9, 2020

Long-term In Vivo Tracking of Inflammatory Cell Dynamics Within Drosophila Pupae

Long-term In Vivo Tracking of Inflammatory Cell Dynamics Within Drosophila Pupae

Published on: June 14, 2018

Microinjection Wound Assay and In vivo Localization of Epidermal Wound Response Reporters in Drosophila Embryos.

Microinjection Wound Assay and In vivo Localization of Epidermal Wound Response Reporters in Drosophila Embryos.

Published on: November 1, 2013

Area of Science:

Cell biology
Developmental biology
Genetics

Background:

Polyploidy, having more than two sets of chromosomes, is common but its role in health and disease is unclear.
Wound-induced polyploidization (WIP) is a conserved tissue repair mechanism across species.
Understanding WIP's origins and functions is crucial.

Purpose of the Study:

To provide a detailed protocol for studying WIP in adult fruit fly epithelium.
To identify genes regulating WIP initiation and function.
To explore factors influencing WIP.

Main Methods:

Utilizing the fruit fly (Drosophila melanogaster) as a model organism.
Developing a protocol to induce and observe WIP in adult fruit fly epithelium post-wounding.
Employing genetic tools to identify regulatory genes.

Main Results:

Polyploid cells are generated within 2 days after puncture wounds in fruit fly epithelium.
The gene Myc has been identified as important for initiating and regulating WIP.
A method for studying WIP in a genetically tractable system is established.

Conclusions:

WIP is a viable and advantageous strategy for tissue repair.
The fruit fly model allows for genetic dissection of WIP.
Further research can elucidate the influence of genetic and physiological factors on WIP.