Related Experiment Video
Updated: Dec 17, 2025

Measuring the Confluence of iPSCs Using an Automated Imaging System
Published on: June 10, 2020
Measuring the Confluence of iPSCs using an Automated Imaging System
Valentina Magliocca1, Maria Vinci2, Tiziana Persichini3
1Genetics and Rare Diseases Research Division, Ospedale Pediatrico Bambino Gesù, IRCCS; Department of Science, University Roma Tre.
Abstract:
This study focuses on understanding how growing iPSCs on different ECM coating substrates can affect cell confluence. A protocol to assess iPSC confluence in real time has been established without the need to count cells in single cell suspension to avoid any growth perturbation. A high-content image analysis system was used to assess iPCS confluence on 4 different ECMs over time in an automated manner. Different analysis settings were used to assess cell confluence of adherent iPSCs and only a slight difference (at 24 and 48 hours with laminin) has been observed whether a 60, 80 or 100% mask was applied. We also show that laminin lead to the best confluence compared to Matrigel, vitronectin and fibronectin.
Related Concept Videos
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview
Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle
The ions and electrons produced interact with the fluctuating magnetic field created by a water-cooled...
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation
There are three main types of inductively coupled plasma atomic emission spectroscopy (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used....

