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Updated: Dec 17, 2025
11:22
Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
30.9K
[Changes in gut microbiota with bronchial asthma].
O Y Zolnikova1, N D Potskhverashvili1, A V Kudryavtseva2
1Sechenov First Moscow State Medical University (Sechenov University).
Terapevticheskii Arkhiv
|June 30, 2020
Summary
Intestinal microbiota differs in bronchial asthma patients, with increased Proteobacteria and altered butyrate-producing bacteria. These changes correlate with asthma severity and suggest a link between gut health and respiratory conditions.
Area of Science:
- Microbiology
- Immunology
- Pulmonology
Background:
- Bronchial asthma (BA) is a chronic respiratory disease with complex pathophysiology.
- The gut microbiome plays a crucial role in immune system modulation and overall health.
Purpose of the Study:
- To investigate alterations in the intestinal microbiota composition of patients with bronchial asthma.
- To explore potential correlations between gut dysbiosis and clinical manifestations of BA.
Main Methods:
- Fecal samples were collected from 40 BA patients and 15 healthy controls.
- 16S rRNA gene sequencing was employed to analyze the gut microbial composition.
Main Results:
- Patients with BA exhibited an increased proportion of Proteobacteria, particularly Betaproteobacteria and Gammaproteobacteria.
- A decrease in butyrate-producing bacteria (e.g., Anaerostipes, Faecalibacterium) and an increase in opportunistic pathogens were observed.
- Specific microbial shifts were associated with allergic vs. non-allergic asthma and the presence of Small Intestinal Bacterial Overgrowth (SIBO).
Conclusions:
- Significant differences exist in the intestinal microflora between healthy individuals and BA patients, including cases with SIBO.
- Gut dysbiosis may contribute to the pathogenesis of bronchial asthma.
- Further research into intestinal bacterial composition is warranted for bronchopulmonary diseases.
Keywords:
bronchial asthmaintestinal microbiotaprobioticssyndrome of intensive bacterial overgrowth in the small intestineMore Related Videos
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