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Related Concept Videos

Effects of EDTA on End-Point Detection Methods01:18

Effects of EDTA on End-Point Detection Methods

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Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
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Related Experiment Video

Updated: Dec 17, 2025

Detection and Removal of Tooth-Colored Composite Resin Using the Fluorescence-Aided Identification Technique
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The assessment of different bleaching agents' efficiency on discoloured teeth using image-processing methods.

Ismail Ozkocak1, Mahmut Hekim2, Hakan Gokturk1

  • 1Bolu Abant Izzet Baysal University, Faculty of Dentistry, Department of Endodontics, Bolu, Turkey.

Photodiagnosis and Photodynamic Therapy
|June 30, 2020
PubMed
Summary

Triple antibiotic paste (TAP) can discolor teeth during regenerative endodontic treatments. Various bleaching agents effectively removed this discoloration, with effectiveness increasing over time. This study evaluated bleaching agents for TAP-induced tooth discoloration.

Keywords:
Image-processingIntracoronal bleachingLaser-activated bleachingRegenerative endodonticsRoot canal medicationTAP

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Area of Science:

  • Endodontics
  • Dental Materials Science
  • Restorative Dentistry

Background:

  • Triple antibiotic paste (TAP) is a common intracanal medicament for regenerative endodontic treatments.
  • TAP has been associated with significant tooth discoloration, posing an aesthetic challenge.
  • Effective bleaching protocols are needed to manage TAP-induced tooth discoloration.

Purpose of the Study:

  • To evaluate the efficacy of different bleaching agents in removing tooth discoloration caused by TAP.
  • To compare the color change (ΔE) achieved by various bleaching agents over time.
  • To assess the suitability of the CIE Lab color system for evaluating bleaching effectiveness.

Main Methods:

  • 120 human maxillary incisors were discolored with TAP for three weeks.
  • Teeth were randomly assigned to five groups: negative control, sodium perborate, Opalescence Endo, Endoperox, and Biolase.
  • Color changes were measured using stereomicroscopy and the CIE Lab color system on days 3 and 7.

Main Results:

  • No significant difference in bleaching efficacy was observed among agents on day 3.
  • On day 7, Biolase showed superior results compared to sodium perborate; other agents showed no significant differences.
  • All tested bleaching protocols demonstrated increased effectiveness over time.

Conclusions:

  • Investigated bleaching protocols can effectively address TAP-induced tooth discoloration.
  • Bleaching effectiveness improves with extended treatment duration.
  • The CIE Lab color system provides a reliable in vitro method for assessing bleaching agent efficiency.