Green synthesis, characterization, antimicrobial and cytotoxic effect of silver nanoparticles using arabinoxylan
Gajendra Nath Maity1, Prasenjit Maity2, Indranil Choudhuri3
1Department of Microbiology, Panskura Banamali College, Panskura, Purba Midnapore Pin-721152, West Bengal, India; Department of Microbiology, Asutosh College, 92, S.P. Mukherjee Road, Kolkata 700026, West Bengal, India.
Abstract:
A green synthesis of silver nanoparticles was synthesized by AgNO3 with arabinoxylan, isolated from green stem of Andrographis paniculata (Kalmegh). The synthesized Ag NPs-arabinoxylan conjugates were characterized by UV-vis spectroscopy, FE-SEM, TEM, XRD, TGA, EDX, and Zeta potential experiments. The Ag NPs formation was established by the surface plasmon resonance band ~410.25 nm. SEM image showed mostly spherical morphology of Ag NPs. The fcc crystalline nature was identified by XRD, SAED and the size were 24.5 and 25 nm from TEM and XRD analysis respectively. The prepared Ag NPs showed dose-dependent antimicrobial activity against Streptococcus pneumonia, Candida albicans and E. coli. The nanoparciles damage 4% hemolysis to human RBCs at 12.5 μg/mL. MTT assay of Ag NPs showed that half of the cell killed at 10 μg/mL and wound healing assay observed effective inhibition cell proliferation.
