Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fluid-induced acid-base variations in postoperative critically ill patients: physiological determinants and renal response.

Frontiers in medicine·2026
Same author

Beyond the calendar: A narrative review on chronobiological drivers of prognosis in resectable NSCLC.

Tumori·2026
Same author

Functional Recovery as a Survivorship Endpoint in Early-Stage NSCLC.

Cancers·2026
Same author

Sugar-mediated physical constraints drive the evolution of pollination drops into nectar.

Scientific reports·2026
Same author

Circulating extracellular vesicles from early-stage lung cancer patients trigger endothelial activation to drive pre-metastatic niche formation through synergistic miR-29a and C4A signaling.

Journal of experimental & clinical cancer research : CR·2026
Same author

Adaptive Plasticity of Phytochelatin Synthase Under Chromium Stress and Sulfur Availability in <i>Scenedesmus acutus</i>.

Plants (Basel, Switzerland)·2026

Related Experiment Video

Updated: Dec 17, 2025

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells
10:33

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells

Published on: June 16, 2019

29.0K

3D profilometry and cell viability studies for drug response screening.

M Carmen Morán1, Francesca Cirisano2, Michele Ferrari3

  • 1Departament de Bioquímica i Fisiologia, Secció de Fisiologia - Facultat de Farmàcia i Ciències de l'Alimentació, Universitat de Barcelona, Avda. Joan XXIII, 27-31, 08028 Barcelona, Spain; Institut de Nanociència i Nanotecnologia - IN(2)UB, Universitat de Barcelona, Avda. Diagonal, 645, 08028 Barcelona, Spain.

Materials Science & Engineering. C, Materials for Biological Applications
|July 1, 2020
PubMed
Summary

Combining noninvasive surface analysis with biochemical methods reveals cell volume changes during proliferation and death. This approach enhances the assessment of drug effects on tumoral versus non-tumoral cells.

Keywords:
3D profilometryApoptosisCell viabilityCell volumeDrug screeningProliferation

More Related Videos

Physiologic Patient Derived 3D Spheroids for Anti-neoplastic Drug Screening to Target Cancer Stem Cells
10:03

Physiologic Patient Derived 3D Spheroids for Anti-neoplastic Drug Screening to Target Cancer Stem Cells

Published on: July 5, 2019

9.2K
Advancements in the Metabolic Profiling of Three-Dimensional Brain Tumor Spheroids for Drug Screening
06:50

Advancements in the Metabolic Profiling of Three-Dimensional Brain Tumor Spheroids for Drug Screening

Published on: September 5, 2025

301

Related Experiment Videos

Last Updated: Dec 17, 2025

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells
10:33

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells

Published on: June 16, 2019

29.0K
Physiologic Patient Derived 3D Spheroids for Anti-neoplastic Drug Screening to Target Cancer Stem Cells
10:03

Physiologic Patient Derived 3D Spheroids for Anti-neoplastic Drug Screening to Target Cancer Stem Cells

Published on: July 5, 2019

9.2K
Advancements in the Metabolic Profiling of Three-Dimensional Brain Tumor Spheroids for Drug Screening
06:50

Advancements in the Metabolic Profiling of Three-Dimensional Brain Tumor Spheroids for Drug Screening

Published on: September 5, 2025

301

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Biophysics

Background:

  • In vitro cell viability assays are crucial for drug and chemical testing but can mask individual cell properties due to high cell numbers.
  • Cellular processes like proliferation and death involve significant volume and surface changes.
  • Existing methods often lack the resolution or non-destructive capabilities to capture these dynamic cellular alterations.

Purpose of the Study:

  • To develop and validate a combined approach using noninvasive morphological surface analysis and biochemical methods.
  • To correlate cell volume variations with cellular viability changes induced by specific treatments.
  • To improve the assessment of drug-induced effects on tumoral versus non-tumoral cells.

Main Methods:

  • Utilized 3D optical scanning profilometry for noninvasive, high-resolution surface and volume analysis at the nano-microscale.
  • Coupled morphological analysis with established biochemical assays for cell viability and response.
  • Administered proliferative and cytotoxic substances to tumoral and non-tumoral cell models.

Main Results:

  • Demonstrated a direct relationship between cell volume changes and cellular viability.
  • Showcased the ability of the combined technique to noninvasively detect and quantify cell volume alterations.
  • Observed improved efficiency in assessing drug-induced changes in tumoral cells compared to non-tumoral cells.

Conclusions:

  • The integration of noninvasive morphological analysis with biochemical methods offers a powerful tool for studying cell viability and drug response.
  • This approach provides enhanced diagnostic features by capturing single-cell morphological dynamics.
  • The method shows particular promise for evaluating the efficacy of chemotherapeutics and other agents on cancer cells.