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Updated: Dec 17, 2025

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
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Downregulation of RUNX3 has a poor prognosis and promotes tumor progress in kidney cancer

Jianbo Zheng1, Yanhui Mei2, Guangsheng Zhai3

  • 1Department of Urology, Qilu Hospital of Shandong University, Jinan, Shandong, China; Department of Urology, Central Hospital of Zibo, Zibo, Shandong, China.

Urologic Oncology
|July 1, 2020
PubMed
Abstract

Insights

Runt-related transcription factor 3 (RUNX3) is downregulated in kidney cancer due to promoter methylation, promoting tumor progression. Restoring RUNX3 may offer new therapeutic strategies for kidney cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Genetics

Background:

  • Kidney cancer often lacks early symptoms and is prone to recurrence.
  • The tumor suppressor role of Runt-related transcription factor 3 (RUNX3) is established in various cancers.

Purpose of the Study:

  • To investigate the role of RUNX3 expression and its promoter methylation in kidney cancer.
  • To explore the potential of RUNX3 as a therapeutic target for kidney cancer.

Main Methods:

  • Quantitative PCR, immunoblotting, and immunohistochemistry were used to assess RUNX3 expression in patient tissues and cell lines.
  • Methylation analysis of the RUNX3 promoter was performed on 103 patient samples.
  • In vitro and in vivo experiments (cell lines, xenograft models) examined the impact of RUNX3 modulation on cancer progression.

Main Results:

  • RUNX3 expression was significantly downregulated in kidney cancer tissues and cells.
  • Increased RUNX3 promoter methylation correlated with decreased RUNX3 expression and poorer kidney cancer prognosis.
  • RUNX3 loss-of-function enhanced, while overexpression inhibited, kidney cancer cell proliferation, migration, and invasion.

Conclusions:

  • RUNX3 acts as a tumor suppressor in kidney cancer, with its activity suppressed by promoter methylation.
  • Targeting the RUNX3 pathway and its promoter methylation presents a promising therapeutic strategy for kidney cancer treatment.
Keywords:
Kidney cancerPTPN7Promoter methylationRUNX3

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