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Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

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Updated: Dec 17, 2025

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

Ultrasensitive amplicon barcoding for next-generation sequencing facilitating sequence error and amplification-bias

Ibrahim Ahmed^1,2, Felicia A Tucci³, Aure Aflalo^1,4

¹Department of Medicine, University of Cambridge, Cambridge, United Kingdom.

Scientific Reports

|July 1, 2020

Summary

Accurate DNA variant proportion analysis is crucial for genetic studies. Our novel ultrasensitive amplicon barcoding method reduces PCR biases and sequencing errors, improving DNA variant proportion estimation.

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Last Updated: Dec 17, 2025

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Published on: August 3, 2018

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

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Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

Area of Science:

Genomics
Molecular Biology
Bioinformatics

Background:

Accurate characterization of DNA variant proportions is essential for various genetic studies.
Challenges include PCR amplification biases and sequencing errors that distort variant frequencies.
Applications span tumor heterogeneity, microbiome, and immune receptor sequencing.

Purpose of the Study:

To develop a novel, generalizable, and ultrasensitive amplicon barcoding approach.
To mitigate the inflation/deflation of DNA variant proportions caused by PCR biases and sequencing errors.
To enhance the quality and diversity estimation in immune receptor sequencing libraries.

Main Methods:

Development of a novel ultrasensitive amplicon barcoding technique.
Application of the method to immune receptor sequencing workflows.
Validation of the approach for reducing PCR amplification biases and sequencing errors.

Main Results:

The developed method significantly reduces distortions in DNA variant proportions.
Improved accuracy in estimating variant frequencies compared to standard methods.
Enhanced quality and diversity estimation of immune receptor sequencing libraries.

Conclusions:

The novel amplicon barcoding approach offers a robust solution for accurate DNA variant proportion analysis.
This method has broad applicability in genetic studies requiring precise variant frequency quantification.
Significant improvements were observed in immune receptor repertoire analysis.