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Labeling of Extracellular Vesicles for Monitoring Migration and Uptake in Cartilage Explants
Published on: October 4, 2021
Engineered-extracellular vesicles as an optimistic tool for microRNA delivery for osteoarthritis treatment
Abazar Esmaeili1,2, Samaneh Hosseini1,3, Mohamadreza Baghaban Eslaminejad4
1Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
Abstract:
Worldwide, osteoarthritis (OA) is one of the most common chronic diseases. In OA, profiling gene expression changes occur and cartilage tissue homeostasis is lost. Suggestions for OA treatment include regulation of gene expressions via the use of microRNAs (miRNAs). However, problems exist with the use of miRNAs, the most important of which is the delivery of sufficient amounts of effective miRNAs to save cartilage tissue. The engineering of extracellular vesicles (EVs) with the use of advanced techniques would be an efficient OA treatment. Therefore, we discuss the importance of miRNAs in terms of cartilage tissue regeneration and review recent advances in production of enriched EVs and miRNA delivery by EVs for future clinical applications.
Insights
Osteoarthritis (OA) involves gene expression changes and cartilage loss. Engineering extracellular vesicles (EVs) offers a promising method for delivering microRNAs (miRNAs) to regenerate cartilage tissue effectively.
Area of Science:
- Biomedical Engineering
- Molecular Biology
- Regenerative Medicine
Background:
- Osteoarthritis (OA) is a prevalent chronic disease characterized by disrupted cartilage homeostasis and altered gene expression.
- Current OA treatments, including microRNA (miRNA)-based therapies, face challenges with effective delivery to target cartilage tissue.
- Extracellular vesicles (EVs) present a potential solution for enhanced miRNA delivery in OA treatment.
Purpose of the Study:
- To highlight the critical role of miRNAs in cartilage tissue regeneration for osteoarthritis.
- To review advancements in engineering extracellular vesicles (EVs) for miRNA enrichment.
- To explore the potential of EV-mediated miRNA delivery for future clinical applications in OA.
Main Methods:
- Review of current literature on miRNA function in osteoarthritis.
- Analysis of recent techniques for producing miRNA-enriched extracellular vesicles.
- Evaluation of extracellular vesicle-based delivery systems for therapeutic miRNAs.
Main Results:
- MicroRNAs play a significant role in regulating gene expression crucial for cartilage homeostasis.
- Advanced engineering techniques enable the production of extracellular vesicles with high miRNA content.
- Extracellular vesicles demonstrate potential as efficient carriers for delivering therapeutic miRNAs to damaged cartilage.
Conclusions:
- MicroRNA-mediated gene regulation is vital for osteoarthritis management.
- Engineered extracellular vesicles offer a viable strategy for overcoming miRNA delivery challenges in OA.
- EV-based miRNA delivery holds promise for future clinical translation in osteoarthritis regenerative therapy.
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