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Dark field microscope-based single nanoparticle identification coupled with statistical analysis for ultrasensitive

Shaohua Xu¹, Longhua Guo², Lifen Chen³

¹Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350116, Fujian, China.

Mikrochimica Acta

|July 1, 2020

Summary

This study introduces a novel method for ultrasensitive ochratoxin A (OTA) detection using gold nanoparticle aggregation visualized by dark field microscopy. The technique offers a simple, efficient, and quantitative approach for identifying OTA at low concentrations.

Keywords:

Aggregation Dark field microscopy Ochratoxin A Single nanoparticle Statistical analysis

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Area of Science:

Nanotechnology
Analytical Chemistry
Biosensing

Background:

Ochratoxin A (OTA) is a prevalent mycotoxin with significant health implications.
Accurate and sensitive detection methods for OTA are crucial for food safety and quality control.
Existing detection methods often require complex procedures or signal amplification.

Purpose of the Study:

To develop a novel, ultrasensitive, and simple method for ochratoxin A (OTA) detection.
To utilize dark field microscopy and gold nanoparticle (AuNP) aggregation for quantitative OTA analysis.
To establish a versatile sensing platform adaptable for other analytes.

Main Methods:

Hybridization of OTA aptamers with single-stranded DNA (DNA1) to create an identification probe.
Induction of gold nanoparticle (AuNP) aggregation upon OTA presence, mediated by DNA1 and DNA2.
Detection and quantification of OTA using dark field microscopy and statistical analysis of AuNP aggregation rates.

Main Results:

The proposed sensor achieved ultrasensitive OTA detection with a limit of detection of 0.1 pg/mL.
A wide detection range from 0.1 pg/mL to 30 ng/mL was demonstrated for OTA.
The method exhibited high efficiency and simplicity, comparable to signal amplification-based sensors.

Conclusions:

The developed dark field microscope-based nanoparticle sensing platform provides a simple, efficient, and ultrasensitive method for OTA detection.
The statistical analysis of AuNP aggregation enables quantitative detection of OTA.
The sensor's design is adaptable for detecting other target molecules by modifying the identification probes.