Nature inspired poly (dopamine quinone -vanadyl) as new modifier for voltammetric determination of uric acid

The preparation of a novel polymer (poly(dopamine quinone-vanadyl) (polyDQV)) bearing dopaminequinone and VO^IV redox groups is described. PolyDQV was characterized using field emission scanning electron microscopy (FESEM), energy dispersive x-ray spectroscopy, x-ray photoelectron spectroscopy (XPS), Fourier transform infra-red (FTIR) spectroscopy, UV-Vis spectroscopy as well as electrochemical methods such as differential pulse voltammetry, cyclic voltammetry, and electrochemical impedance spectroscopy (EIS). The electrocatalytic activity of polyDQV was studied toward electrooxidation of uric acid using differential pulse voltammetry as well as cyclic voltammetry. PolyDQV presents interesting electrocatalytic activity toward UA oxidation in phosphate buffer solution (0.1 M, pH 2) to a well-defined oxidation peak at 0.65 V (vs. Ag/AgCl). The polyDQV-modified carbon paste electrode (CPE/polyDQV) presents a precise linear signal-concentration relationship in the ranges of 0.3-5 μM and 5 to 200 μM with a detection limit (S/N = 3) of 0.02 μM. The %RSD values for ten replicate measurements of 0.5 and 50 μM UA were 1.8 and 3%, respectively, indicating good repeatability of analytical signals. Appropriate recovery values (in the range 96 to 103%) and good selectivity for UA over common coexisting species (such as ascorbic acid and dopamine) exhibit that CPE/polyDQV is a promising novel platform for sensing UA in human blood serum and urine samples. Graphical abstract.