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Differential expression of HLA class-I antigens on B and T lymphocytes obtained from human lymphoid tissues

A Bishara1, D Nelken, C Brautbar

  • 1Department of Clinical Microbiology, Hebrew University-Hadassah Medical Center, Jerusalem, Israel.

Immunobiology
|April 1, 1988
PubMed

The amount of HLA class-I antigens was determined on the surface of enriched populations of B and T lymphocytes obtained from human peripheral blood, lymph nodes and spleens. The assays were performed using monoclonal antibodies that recognize different determinants on HLA class-I antigens and utilizing a simple and sensitive enzyme-linked immunosorbent assay (ELISA). Our results indicate that B lymphocytes obtained from human peripheral blood, lymph nodes and spleens express at least twice as many HLA class-I antigens as T lymphocytes obtained from the same organs.

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