Related Experiment Video
Updated: Dec 17, 2025

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains
Published on: November 30, 2021
Estimation of adult age-at-death from entheseal robusticity: A test using an identified Italian skeletal collection
Marco Milella1, Maria Giovanna Belcastro2, Valentina Mariotti2
1Department of Physical Anthropology, Institute of Forensic Medicine, University of Bern, Bern, Switzerland.
Objectives:
The present study tests the effectiveness of entheseal robusticity (ER) as a potential predictor of adult age-at-death by applying multiple regression models to a large contemporary identified skeletal sample.
Materials And Methods:
ER was recorded for 23 bilateral postcranial entheses on 481 adult individuals (271 females and 210 males) from the Frassetto identified skeletal collection of Sassari (Italy), following the method of Mariotti et al. 2007. ER scores were used as predictors in multiple regression analyses with age as the dependent variable; the performance of the resulting models was tested through standard error of estimate, the correlation coefficient between predicted and documented age, and the percentage of correctly classified individuals.
Results:
ER show a higher correlation with age in females, resulting in more accurate estimates when compared with those obtained for males. Age-at-death is overestimated for male individuals under 45 years old and underestimated for older individuals. Regression models including a reduced set of ER sites result in better estimates compared with those using the complete set of variables.
Discussion:
Results suggest the potential usefulness of ER for the estimation of age, especially for female skeletal remains. If used with caution, ER may be a valid complement to existing methods for the reconstruction of the biological profile of skeletal remains of archaeological and forensic interest.
Related Concept Videos
Changes in the Appendicular Skeleton with Age
Initially, the limb buds consist of a core of mesenchyme covered by a layer of ectoderm. The ectoderm at the end of the limb bud thickens to form a narrow crest called the apical ectodermal ridge. This ridge stimulates the underlying...
Classification of Bones
Long and Short Bones
The appendicular skeleton, particularly the upper and lower limbs, is primarily made of long and short bones. The...
Gross Anatomy of Bone
The diaphysis is the tubular shaft that runs between the proximal and distal ends of the bone. The walls of the diaphysis are composed of dense and hard compact bone made of numerous osteons — the functional unit of the compact bone. The hollow region in the diaphysis is called the medullary cavity, which harbors the bone marrow. In infants and children, this marrow cavity is filled with red marrow, whereas in...

