Related Experiment Video
Updated: Aug 4, 2026

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Current knowledge on the human eye microbiome: a systematic review of available amplicon and metagenomic sequencing
Heleen Delbeke1, Saif Younas2, Ingele Casteels1
1Department of Ophthalmology, University Hospitals Leuven, Leuven, Belgium.
Abstract:
Insights in the ocular surface microbiome are still at an early stage and many more questions remain unanswered compared with other human-associated microbial communities. The current knowledge on the human microbiome changed our viewpoint on bacteria and human health and significantly enhanced our understanding of human pathophysiology. Also in ocular medicine, microbiome research might impact treatment. Here, we summarize the current knowledge on ocular microbiome research with a particular focus on potential confounding factors and their effects on microbiome composition. Moreover, we present the ocular surface core microbiome based on current available data and defined it as genera present in almost half of the published control cohorts with a relative abundance of at least 1%.
Related Concept Videos
Methods to Assess Microbial Communities
Introduction to the Human Microbiota
Microbiome of the Eye
The Oral Microbiota

