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Related Concept Videos

MicroRNAs01:22

MicroRNAs

3.6K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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Related Experiment Video

Updated: Dec 17, 2025

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method
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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method

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Multiplex Digital MicroRNA Detection Using Cross-Inhibitory DNA Circuits.

Yannick Rondelez1, Guillaume Gines1

  • 1Gulliver Laboratory, ESPCI Paris-Université PSL, 10 rue Vauquelin, 75005 Paris, France.

ACS Sensors
|July 1, 2020
PubMed
Summary
This summary is machine-generated.

This study introduces a novel method for simultaneously detecting multiple microRNAs (miRNAs) using a single-step, isothermal process. The technique overcomes limitations of existing methods, enabling sensitive and specific digital biomarker analysis for clinical applications.

Keywords:
DNA circuitdigital droplet detectionisothermal amplificationmicroRNAmultiplex assay

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Biomarker Discovery

Background:

  • MicroRNAs (miRNAs) are crucial post-transcriptional regulators and emerging biomarkers for physiological and pathological states.
  • Multiplex and digital detection of miRNAs is essential for clinical applications but faces significant challenges.
  • Current methods like RT-PCR have limitations including thermocycling and multi-step processes, while isothermal alternatives lack sensitivity or suffer from cross-talk.

Purpose of the Study:

  • To develop a sensitive, isothermal, and multiplex method for digital microRNA detection.
  • To overcome the limitations of existing miRNA quantification techniques, particularly molecular cross-talk and sensitivity issues.
  • To enable robust and simultaneous detection of multiple microRNA targets in a single reaction.

Main Methods:

  • Development of an ultrasensitive isothermal amplification mechanism.
  • Integration of target-specific DNA circuits with DNA-encoded inhibitors to suppress nonspecific amplification.
  • Design of a one-step, isothermal assay for digital and multiplex microRNA quantification.

Main Results:

  • Successful suppression of molecular cross-talk, enabling specific amplification.
  • Demonstration of ultrasensitive, single-molecule detection capabilities.
  • Achieved one-step, isothermal, digital, and simultaneous quantification of multiple microRNA targets.

Conclusions:

  • The developed strategy provides a robust platform for isothermal, multiplex digital detection of microRNAs.
  • This approach addresses key challenges in miRNA biomarker analysis, paving the way for clinical utility.
  • The method offers a simplified, sensitive, and specific alternative for miRNA quantification.