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Updated: Dec 17, 2025
Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes
Published on: September 19, 2013
Chemiluminescence assay for Listeria monocytogenes based on Cu/Co/Ni ternary nanocatalyst coupled with penicillin as
Yue Zhang1, Gaoxi Cui1, Yuchan Meng1
1Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE; Shandong Key Laboratory of Biochemical Analysis; Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong; College of Marine Science and Biological Engineering; Institute of Life Sciences and Biomass Resources; College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, China.
Abstract:
Bacterial pathogen control is important in seafood production. In this study, a Cu/Co/Ni ternary nanoalloy (Cu/Co/Ni TNA) was synthesized using the oleylamine reducing method. It was found that Cu/Co/Ni TNA greatly enhanced the chemiluminescence (CL) signal of the hydroxylamine-O-sulfonic acid (HOSA)-luminol system. The CL properties of Cu/Co/Ni TNA were investigated systemically. The possible CL mechanism also was intensively investigated. Based on the enhanced CL phenomenon of Cu/Co/Ni TNA, a Cu/Co/Ni TNA, penicillin, and anti-L. monocytogenes (Listeria monocytogenes) antibody-based sandwich complex assay for detection of L. monocytogenes was established. In this sandwich CL assay, penicillin was employed to capture and enrich pathogenic bacteria with penicillin-binding proteins (PBPs) while anti-L. monocytogenes antibody was adopted as the specific recognition molecule to recognize L. monocytogenes. L. monocytogenes was detected sensitively based on this new Cu/Co/Ni TNA-HOSA-luminol CL system. The CL intensity was proportional to the L. monocytogenes concentration ranging from 2.0 × 102 CFU ml-1 to 3.0 × 107 CFU ml-1 and the limit of detection wa 70 CFU ml-1 . The reliability and potential applications of our method was verified by comparison with official methods and recovery tests in environment and food samples.
