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Related Experiment Video

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Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice
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A method to improve embryo development potential when fertilization is delayed in mice.

Dapeng Chu1, Haiping Wang1, Lei Fu1

  • 1Medical Center for Human Reproduction, Beijing Chao-Yang Hospital, Capital Medical University , Beijing, People's Republic of China.

Systems Biology in Reproductive Medicine
|July 1, 2020
PubMed
Summary
This summary is machine-generated.

Adding 5mM glucose to fertilization media significantly improved mouse embryo development and birth rates when fertilization was delayed by 6 hours. This enhancement did not negatively impact standard in vitro fertilization (IVF) outcomes, suggesting potential clinical applications in assisted reproductive technology (ART).

Keywords:
Delayed fertilizationartembryo development potentialoocyte aging

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Area of Science:

  • Reproductive biology
  • Developmental biology
  • Assisted reproductive technology (ART)

Background:

  • Delayed fertilization can negatively impact embryo development potential.
  • Optimizing fertilization media is crucial for improving outcomes in assisted reproductive technology (ART).

Purpose of the Study:

  • To investigate the effect of glucose supplementation in fertilization media on mouse embryo development with delayed fertilization.
  • To assess the impact of different glucose concentrations (5mM and 10mM) on blastocyst rates and subsequent birth outcomes.

Main Methods:

  • Mouse oocytes were cultured for 6 hours before fertilization in media with varying glucose concentrations (0mM, 5mM, 10mM).
  • Fertilization was performed, and embryos were cultured to the blastocyst stage.
  • Embryo transfer was conducted, and birth outcomes were evaluated for both delayed and conventional in vitro fertilization (IVF) protocols.

Main Results:

  • Supplementation with 5mM glucose significantly increased blastocyst and birth rates in cases of 6-hour delayed fertilization compared to controls (p < 0.05).
  • In conventional IVF without fertilization delay, 5mM and 10mM glucose did not significantly affect embryo development.
  • No significant differences were observed in birth rate, fetal weight, or birth defect rates among the groups.

Conclusions:

  • 5mM glucose supplementation effectively enhances embryo developmental potential and birth outcomes when fertilization is delayed by 6 hours.
  • Glucose supplementation at 5mM does not adversely affect embryo quality or birth outcomes in standard IVF procedures.
  • This finding suggests a promising clinical application for 5mM glucose in assisted reproductive technology (ART) protocols involving delayed fertilization.