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Updated: Dec 16, 2025
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Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa
Published on: June 30, 2016
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Cyclic di-AMP Signaling in Bacteria
1Department of General Microbiology, Göttingen Center for Molecular Biosciences (GZMB), Georg-August-University Göttingen, 37077 Göttingen, Germany;
Annual Review of Microbiology
|July 1, 2020
Summary
Cyclic di-AMP (c-di-AMP), a vital molecule for bacterial survival on rich media, regulates essential cellular processes. Its importance stems from multilevel control of homeostasis, not a single target.
Area of Science:
- Microbiology
- Molecular Biology
- Biochemistry
Background:
- Cyclic di-AMP (c-di-AMP) is a ubiquitous second messenger in bacteria and archaea.
- This molecule is essential for the viability of many species when grown on rich media.
- c-di-AMP interacts with numerous proteins and riboswitches, primarily influencing potassium and osmotic homeostasis.
Purpose of the Study:
- To review the current understanding of c-di-AMP homeostasis.
- To summarize the functions of c-di-AMP in controlling cellular processes.
- To elucidate the multilevel regulatory roles of c-di-AMP in bacterial physiology.
Main Methods:
- Literature review of recent research on c-di-AMP.
- Analysis of studies on protein and riboswitch binding.
- Examination of c-di-AMP's role in osmotic and potassium homeostasis.
Main Results:
- c-di-AMP is dispensable in minimal media with low osmotic compounds.
- The essentiality of c-di-AMP is attributed to its role in complex homeostatic processes.
- c-di-AMP regulates multiple cellular functions through diverse molecular interactions.
Conclusions:
- c-di-AMP is crucial for bacterial survival through intricate homeostatic regulation.
- Understanding c-di-AMP homeostasis provides insights into bacterial adaptation and survival strategies.
- The review highlights the significance of c-di-AMP as a key regulator of cellular processes.
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