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Updated: Dec 16, 2025
Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
Published on: December 11, 2021
A flexible tripod fluorescent probe for multiple cations detection and its application in living cells
Wan-Li Xu1, Jun-Feng Yang1, Mao-Qian Ran1
1Key Laboratory of Macrocyclic and Supramolecular Chemistry of Guizhou Province, School of Chemistry and Chemical Engineering, Guizhou University, Guiyang 550025, China.
Abstract:
To the flexible tripod platform tren (tris(2-aminoethyl)amine), a Rhodamine and two naphthalene fluorophores were introduced. The resulting fluorescence probe named TRN was fully characterized and employed in cell imaging. Probe TRN exhibited high selectivity and excellent sensitivity for the simultaneous fluorescence detection of Zn2+/Hg2+/Al3+/Cu2+. The significant changes in the fluorescence color make naked-eye detection possible. Furthermore, fluorescence imaging experiments of Zn2+/Hg2+/Al3+/Cu2+ in living PC3 cells demonstrated its value for practical applications in biological systems.
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