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A benchmarking protocol for breath analysis: the peppermint experiment.

Ben Henderson1, Dorota M Ruszkiewicz, Max Wilkinson

  • 1Exhaled Biomarkers and Exposure, Department of Molecular and Laser Physics, IMM, Radboud University, Nijmegen, The Netherlands.

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Summary
This summary is machine-generated.

The Peppermint Initiative establishes a standardized method for analyzing volatile organic compounds (VOCs) in breath, improving disease detection reproducibility. This approach provides benchmark values for breath sampling and data analysis, crucial for consistent results.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Respiratory Medicine

Background:

  • Standardization is lacking in volatile organic compound (VOC) analysis for disease detection.
  • Replicability of results is hindered by inconsistent breath sampling and data analysis methods.
  • The Peppermint Initiative aims to address these limitations through a standardized experimental approach.

Purpose of the Study:

  • To disseminate a standardized breath sampling and analysis experiment for VOCs.
  • To establish benchmark values for VOC measurements in exhaled breath.
  • To enable comparison of different breath sampling and data analysis techniques.

Main Methods:

  • A standardized intervention using peppermint oil capsules to alter breath VOC profiles.
  • Breath sampling at pre-ingestion and 2, 4, 6, 8, 10 hours post-ingestion.
  • Analysis using ion mobility spectrometry, GC-MS, and regression analysis for washout profiles.

Main Results:

  • Pilot data illustrate a standardized interpretation of results from the Peppermint experiment.
  • Washout profile data determined optimal sampling time points and established a benchmark value.
  • A data fidelity measure was proposed to assess data quality from different techniques.

Conclusions:

  • The Peppermint Initiative provides a standardized protocol for breath VOC analysis.
  • This standardized approach facilitates comparison across different sampling and analytical platforms.
  • The initiative has collected extensive data from multiple partners, paving the way for future dissemination.