Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Euchromatin01:01

Euchromatin

8.7K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
8.7K
Heterochromatin02:38

Heterochromatin

17.6K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
17.6K
Chromatin Packaging02:21

Chromatin Packaging

20.8K
Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter? 
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order...
20.8K
Chromatin Packaging01:32

Chromatin Packaging

18.6K
Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...
18.6K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

1.4K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
1.4K
Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

7.1K
The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
7.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

XL-MS-Guided Structure Prediction of Disordered <i>Encephalitozoon hellem</i> Proteins.

bioRxiv : the preprint server for biology·2026
Same author

Single-molecule imaging reveals RNA polymerase II dynamics and TAF1-dependent promoter-proximal pause release.

Nature communications·2026
Same author

ATF4 coordinates amino acid and nucleotide synthesis with selective protein translation to ensure proper DNA replication timing in leukemia cells.

Nature communications·2026
Same author

Histone succinylation directly inhibits Jumonji domain demethylases and stabilizes repressive chromatin states.

bioRxiv : the preprint server for biology·2026
Same author

The GNMT N-terminus Couples Folate Feedback to Methyl-donor Homeostasis.

bioRxiv : the preprint server for biology·2026
Same author

Multi-omics analyses identify EZH2 as a central driver in rhabdomyosarcoma radioresistance and highlight Tazemetostat as an effective radiosensitizer in vitro and in vivo.

Cell death & disease·2026

Related Experiment Video

Updated: Dec 16, 2025

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
24:02

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

Published on: April 11, 2014

18.6K

Mass Spectrometry to Study Chromatin Compaction.

Stephanie Stransky1, Jennifer Aguilan2, Jake Lachowicz1

  • 1Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.

Biology
|July 2, 2020
PubMed
Summary
This summary is machine-generated.

Emerging mass spectrometry methods quantify chromatin accessibility, revealing protein roles in gene regulation. This approach enhances understanding of chromatin states in aging, infection, and cancer.

Keywords:
DNA methylationchromatinhistonemass spectrometrypost-translational modificationproteome

More Related Videos

Author Spotlight: Enhanced Histone PTM Isomer Identification Through LC-TIMS-ToF MS/MS and PASEF
05:52

Author Spotlight: Enhanced Histone PTM Isomer Identification Through LC-TIMS-ToF MS/MS and PASEF

Published on: January 12, 2024

1.5K
Quantitative Analysis of Chromatin Proteomes in Disease
08:11

Quantitative Analysis of Chromatin Proteomes in Disease

Published on: December 28, 2012

13.5K

Related Experiment Videos

Last Updated: Dec 16, 2025

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
24:02

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

Published on: April 11, 2014

18.6K
Author Spotlight: Enhanced Histone PTM Isomer Identification Through LC-TIMS-ToF MS/MS and PASEF
05:52

Author Spotlight: Enhanced Histone PTM Isomer Identification Through LC-TIMS-ToF MS/MS and PASEF

Published on: January 12, 2024

1.5K
Quantitative Analysis of Chromatin Proteomes in Disease
08:11

Quantitative Analysis of Chromatin Proteomes in Disease

Published on: December 28, 2012

13.5K

Area of Science:

  • Molecular Biology
  • Genomics
  • Proteomics

Background:

  • Chromatin accessibility regulates gene expression, with histone modifications playing a key role.
  • Dysfunctional chromatin condensation is linked to aging, viral infections, and approximately 50% of cancers.
  • Existing genomics methods for chromatin state analysis lack a protein-centric perspective.

Purpose of the Study:

  • To provide an overview of current chromatin accessibility data generation methods.
  • To highlight emerging mass spectrometry-based approaches for quantifying chromatin-bound proteomes and histone accessibility.
  • To emphasize the potential of proteomics in advancing chromatin biology research.

Main Methods:

  • Review of established genomics techniques for chromatin state analysis.
  • Focus on mass spectrometry (MS) for large-scale, unbiased quantification of chromatin proteomes.
  • Application of MS to assess protein and histone modification accessibility on chromatin.

Main Results:

  • Mass spectrometry offers an unbiased, large-scale method for proteome quantification.
  • Chromatin accessibility analysis via MS adds a quantitative layer to proteomics data.
  • This approach facilitates data-driven hypotheses in chromatin biology.

Conclusions:

  • Emerging MS methods provide a protein-level view of chromatin accessibility.
  • These techniques can enhance predictive power regarding critical proteins and histone modifications in gene regulation.
  • Understanding protein occupancy in different chromatin states is crucial for health and disease insights.