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Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

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Multiplexed High-Throughput Serological Assay for Human Enteroviruses.

Niila V V Saarinen¹, Jussi Lehtonen¹, Riitta Veijola²

¹Faculty of Medicine and Health Technology, Tampere University, 33520 Tampere, Finland.

|July 2, 2020

Summary

This study developed a multiplex assay to detect antibodies against multiple enterovirus and rhinovirus types. The new assay provides more precise evaluation of humoral immunity in children compared to single-type assays.

Keywords:

enterovirus multiplex immunoassay rhinovirus

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Area of Science:

Virology and Immunology
Infectious Diseases
Assay Development

Background:

Current immunological assays for enteroviruses often rely on single serotypes, limiting comprehensive antibody response detection.
This limitation hinders the assessment of type-specific antibody variations and broad immunity against diverse enterovirus and rhinovirus types.

Purpose of the Study:

To develop a multiplexed assay for simultaneous detection of antibodies against multiple enterovirus and rhinovirus types.
To compare humoral immune responses to enteroviruses and rhinoviruses in adults and children using the novel assay.

Main Methods:

Production of seven recombinant VP1 proteins from enterovirus (EV-A to EV-D) and rhinovirus (RV-A to RV-C) species.
Utilized the Meso Scale Diagnostics U-PLEX platform for simultaneous antibody detection in 140 human serum samples.
Analyzed antibody reactions against viral proteins and non-structural enterovirus proteins in a single assay well.

Main Results:

Adults exhibited significantly stronger antibody responses (33-fold) compared to children (p < 10^-11).
Children demonstrated reduced cross-reactivity between different enterovirus types compared to adults.
The multiplex assay successfully detected antibodies against multiple enterovirus and rhinovirus types.

Conclusions:

The developed high-throughput multiplex assay offers enhanced evaluation of humoral enterovirus immunity.
This assay provides more exact immunological information in children than traditional single-type assays.
The findings highlight the utility of multiplex assays for understanding complex viral immune responses.