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Association Between HMGB1 and Thrombogenesis in a Hyperlipaemia-induced Microminipig Model of Atherosclerosis
Satoru Kake1,2, Hiroaki Kawaguchi3, Tomoka Nagasato4,5
1Laboratory of Veterinary Pharmacology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.
Background/Aim:
An appropriate animal model is essential to investigate the relationship between inflammation, atherosclerosis, and thrombogenesis, and the development of preventive measures and therapies for atherosclerosis.
Materials And Methods:
Atherosclerosis was induced in Microminipigs (MMPs) using a high-fat diet. We assessed high mobility group box 1 (HMGB1) expression levels and measured thrombus formation using a Total Thrombus Formation Analysis System (T-TAS). MMPs were divided into a normal diet (control) group and four high-fat diet groups, with differing amounts of cholesterol. After 8 weeks, blood was collected for analysis.
Results:
HMGB1 levels increased with increasing dietary cholesterol, and a negative correlation was found between HMGB1 levels and thrombus formation time.
Conclusion:
T-TAS is useful in the assessment of thrombogenesis in MMPs and HMGB1 is associated with thrombus formation.
Insights
Microminipigs fed high-cholesterol diets showed increased high mobility group box 1 (HMGB1) levels, which correlated with reduced thrombus formation time. This suggests HMGB1
Area of Science:
- Cardiovascular Research
- Animal Models of Disease
- Inflammation and Thrombosis
Background:
- Atherosclerosis research requires suitable animal models to study inflammation, atherosclerosis, and thrombogenesis.
- Developing effective preventive measures and therapies for atherosclerosis is crucial.
Purpose of the Study:
- To evaluate Microminipigs (MMPs) as an animal model for atherosclerosis.
- To investigate the relationship between high mobility group box 1 (HMGB1) and thrombus formation in MMPs.
Main Methods:
- Atherosclerosis was induced in MMPs using a high-fat diet with varying cholesterol levels.
- High mobility group box 1 (HMGB1) expression and thrombus formation were measured using Total Thrombus Formation Analysis System (T-TAS).
Main Results:
- HMGB1 levels increased proportionally with dietary cholesterol.
- A negative correlation was observed between HMGB1 levels and thrombus formation time, indicating faster clotting.
Conclusions:
- The Total Thrombus Formation Analysis System (T-TAS) is effective for assessing thrombogenesis in MMPs.
- High mobility group box 1 (HMGB1) is significantly associated with thrombus formation in this atherosclerosis model.
