Liver Macrophage Isolation by Flow Cytometry Sorting
1Department of Medicine, Integrated Cardio Metabolic Center, Karolinska Institutet, Huddinge, Sweden. andre.sulen.2@ki.se.
Methods in Molecular Biology (Clifton, N.J.)
|July 2, 2020
Abstract:
Liver macrophages are immune cells that under steady state conditions play important roles in maintaining tissue homeostasis. But these cells are also intricately linked to the pathogenesis of obesity-related diseases such as nonalcoholic fatty liver disease (NAFLD), nonalcoholic steatohepatitis (NASH), and type 2 diabetes (T2D). Described herein is a protocol to isolate liver macrophages by FACS for downstream molecular analysis. Such studies of macrophages are important for elucidating their role in the pathogenesis of these metabolic diseases.
