Related Experiment Video
Updated: Dec 16, 2025

Author Spotlight: Illuminating New Avenues for Adipose Tissue Metabolism and Disease Prevention
Published on: October 6, 2023
Subcellular Compartmentation of Metabolites Involved in Cold Acclimation
Imke I Hoermiller1, Thomas Nägele2, Arnd G Heyer3
1Department of Plant Biotechnology, Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, Stuttgart, Germany.
Abstract:
Plant cells are heavily compartmentalized, and metabolite concentrations in the various compartments differ significantly. Thus, determination of metabolite abundance in whole-cell extracts may be misleading, when the role of a compound in plant freezing tolerance shall be evaluated. Here, we describe a method for the separation of the largest compartments of plant cells, the vacuole, plastid, and cytosol. With more elaborate analysis, this method can be expanded to also resolve mitochondria and other compartments.
More Related Videos
08:07Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage
Published on: September 16, 2025
10:28Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics
Published on: October 19, 2018
Related Concept Videos
Responses to Heat and Cold Stress
Eukaryotic Compartmentalization
For example, lysosomes in the animal...
Eukaryotic Compartmentalizations
For example, lysosomes in the animal cells...
Subcellular Fractionation
Differential Centrifugation
Differential centrifugation is...
Cell Inclusions
Protein Transport to the Inner Chloroplast Membrane