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Updated: Dec 16, 2025

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

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Published on: March 30, 2018

Brassinosteroids regulate root meristem development by mediating BIN2-UPB1 module in Arabidopsis.

Taotao Li¹, Wei Lei¹, Ruiyuan He¹

¹Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, State Key Laboratory of Hydraulics and Mountain River Engineering, College of Life Sciences, Sichuan University, Chengdu, Sichuan, P. R. China.

|July 2, 2020

Summary

Brassinosteroids (BRs) promote root growth by inhibiting the BIN2-UPB1 module. BIN2 phosphorylates UPB1, enhancing its activity and suppressing root development.

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Area of Science:

Plant biology
Molecular plant science
Plant hormones

Background:

Brassinosteroids (BRs) are crucial plant steroid hormones regulating growth and development.
Negative regulators in the root meristem that BRs counteract are not fully characterized.
The role of UPB1 transcription factor in BR-regulated root growth requires elucidation.

Purpose of the Study:

To investigate the molecular mechanism of BR-mediated root growth regulation.
To determine the function of the UPB1 transcription factor in BR signaling pathways.
To identify interactions between BR signaling components and root development regulators.

Main Methods:

Protein-protein interaction assays (BIN2-UPB1).
Phosphorylation site analysis and mutagenesis (UPB1S37AS41A).
Genetic analysis of BR-deficient and bin2-1 mutants.
Transcriptional activity assays and protein interaction studies (UPB1-PRE2/3).

Main Results:

BIN2 phosphorylates UPB1, increasing its stability and transcriptional activity.
UPB1 deficiency partially rescues the short-root phenotype in BR-deficient mutants.
A non-phosphorylatable UPB1 mutant rescues the short-root phenotype of bin2-1.
UPB1 is transcriptionally repressed by BES1 and its interaction with PRE2/3 is inhibited by BIN2-mediated phosphorylation.

Conclusions:

BRs promote root growth by inhibiting the BIN2-UPB1 signaling module.
BIN2-mediated phosphorylation of UPB1 is a key step in suppressing root meristem development.
This study elucidates a novel molecular mechanism controlling BR-regulated plant growth.