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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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Related Experiment Video

Updated: Dec 16, 2025

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web

Published on: July 16, 2017

Beyond protein structure determination with MicroED.

Chi Nguyen¹, Tamir Gonen²

¹Department of Biological Chemistry, University of California Los Angeles, 615 Charles E Young Drive South, Los Angeles, CA90095, United States.

Current Opinion in Structural Biology

|July 2, 2020

Summary

This summary is machine-generated.

Microcrystal electron diffraction (MicroED) advances cryo-electron microscopy for determining structures of challenging molecules. This technique is expanding its impact beyond proteins to diverse chemical and natural product analyses.

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Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

Area of Science:

Structural biology
Materials science
Chemistry

Background:

Microcrystal electron diffraction (MicroED) emerged in 2013 as a novel cryo-electron microscopy (cryoEM) technique.
MicroED has significantly improved the resolution of protein structure determination using cryoEM.
The technique has also enabled structure determination for molecules previously resistant to other methods.

Purpose of the Study:

To review recent advancements in MicroED technology and applications.
To highlight the expanding role of MicroED beyond protein structure analysis.
To showcase the paradigm-shifting potential of MicroED in diverse scientific fields.

Main Methods:

Utilizes microcrystal electron diffraction (MicroED) for structural analysis.
Applies electron cryomicroscopy (cryoEM) principles.
Focuses on structure determination of peptides, small organic/inorganic molecules, and natural products.

Main Results:

Demonstrates MicroED's capability in high-resolution structure determination.
Shows successful application to a wide range of molecules, including those difficult for other techniques.
Highlights recent developments expanding MicroED's utility and impact.

Conclusions:

MicroED is a powerful and versatile technique for molecular structure determination.
Its applications are rapidly growing beyond traditional protein crystallography.
MicroED is poised to revolutionize structural studies in chemistry, materials science, and beyond.