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Related Experiment Videos

Coagulation activities of plasma microparticles.

M A Howard1, M Coghlan, R David

  • 1Department of Medicine, Monash Medical School, Prahran, Victoria, Australia.

Thrombosis Research
|April 1, 1988
PubMed
Summary

Plasma microparticles (MPs) significantly impact in vitro coagulation tests, shortening activated partial thromboplastin time (APTT) and dilute simplastin time (DSTT). These MPs, distinct from commercial phospholipids, influence routine coagulation assays.

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Area of Science:

  • Hematology
  • Biochemistry
  • Coagulation Science

Background:

  • Plasma microparticles (MPs) are known to influence hemostasis.
  • Their precise contribution to routine coagulation assays requires further elucidation.

Purpose of the Study:

  • To evaluate the effect of plasma microparticles (MPs) on in vitro coagulation.
  • To differentiate the coagulant activity of MPs from commercial phospholipid preparations.

Main Methods:

  • Preparation of platelet-rich (PRP), platelet-poor (PPP), and platelet-free (PFP) plasmas via differential centrifugation.
  • Assessment of activated partial thromboplastin time (APTT) and dilute simplastin time (DSTT).
  • Analysis of Factor VIII (F.VIII:C) and von Willebrand factor (vWf) association with MPs using sucrose gradients.

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Main Results:

  • MPs significantly shortened APTT and DSTT, independent of commercial phospholipids.
  • Platelet factor 3 (PF3) availability correlated with centrifugation force and was abundant in the MP fraction.
  • Factor VIII (F.VIII:C) and von Willebrand factor (vWf) were associated with MPs but separable.
  • The procoagulant effect of MPs on APTT was independent of F.VIII:C/vWf and not solely due to PF3.

Conclusions:

  • Plasma microparticles (MPs) are integral components of routine coagulation assays, affecting APTT and DSTT.
  • High-speed centrifugation reduces F.VIII:C and vWf levels, impacting protein recovery during plasma preparation.