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Updated: Dec 16, 2025
Establishing a Mouse Model of Thin Endometrium
Published on: November 1, 2024
The impact of granulocyte colony-stimulating factor (G-CSF) on thin endometrium of an animal model with rats
1Department of Obstetrics and Gynecology, Gazi University School of Medicine, Besevler, Ankara, Turkey.
Materials And Methods:
After forming of the thin endometrium by uterine injection of 0.2 ml 96% ethyl alcohol to the rats, five days of subcutaneous injections of 40 μg/kg G-CSF or saline were given. Endometrial thickness, immunohistochemically expression of vascular endothelial growth factor receptor-2 (VEGF-R2), proliferative cell nuclear antigen (PCNA) and fibronectin apoptosis with TUNEL method were compared in specimens among four groups of post-model rats.
Results:
Endometrial thickness was significantly improved in thin but not in normal endometrium group with GCSF when compared to saline injection. Stromal and glandular epithelial expression of PCNA and pericapillary VEGF-R2 was significantly increased, and apoptosis was significantly decreased with G-CSF. Although fibronectin was also increased with G-CSF in the thin endometrium, the difference was non-significant. In further, G-CSF decreased apoptotic cells and increased expression of PCNA when compared to saline injection in normal endometrium.
Conclusions:
G-CSF improves endometrial thickness, proliferation, angiogenesis and DNA fragmentation in thin endometrium.
