Two Mixed-ligand Coordination Polymers: Crystal Structures and Protective Effect on Ischemic Stroke by Increasing glp1r Expression

In this study, two new mixed-ligand coordination polymers {[Co(bbi)(tdc)]·5H₂O}_n (1, bbi = 1,4-bis(imidazolyl)butane) and {[Cu₂(bimb)(H₂O)(μ₃-tdc)₂(DMF)₂]·H₂O}_n (2, bimb = 4-bis(1H-imidazol-1-yl-methyl)benzene) were synthesized under the solvothermal conditions via reaction of 2,5-thiophenedicarboxylic acid (H₂tdc) with the corresponding metal salts in the existence of different flexible bis-imidazole ligands (bbi for 1 and bimb for 2). The as-prepared two structures were detected via the single crystal X-ray diffraction (SXRD) and then characterized via the analysis of element, powder X-ray diffraction (PXRD), thermogravimetric analysis (TGA) as well as infrared (IR) spectroscopy. Furthermore, the protective activity of the compound on the mice with ischemic stroke was evaluated. Firstly, the real time reverse transcription-polymerase chain raction (RT-PCR) was carried out to determine the effect of compounds 1 and 2 against the relative expression level of the glucagon-like peptide 1 receptors (glp1r) on the cerebrovascular endothelial cells. Next, the Morris Water Maze Experiment was also used to detect the improvement function of compounds 1 and 2 on the nice mice cognitive function.