Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Complement conversion and leukocyte kinetics in open heart surgery.

J Utoh1, T Yamamoto, T Kambara

  • 1First Department of Surgery, Kumamoto University Medical School, Japan.

The Japanese Journal of Surgery
|May 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[A surgically treated case of ventrally exophytic pontine glioma].

No shinkei geka. Neurological surgery·1995
Same author

[Anomalous origin of the right pulmonary artery from the ascending aorta: a case report].

Kyobu geka. The Japanese journal of thoracic surgery·1995
Same author

Response of human gingival fibroblasts to prostaglandins.

Journal of periodontal research·1995
Same author

Hemodynamic effects of bolus nicorandil compared with nitroglycerin.

The American journal of emergency medicine·1995
Same author

Endovascular approach for an intracranial mycotic aneurysm associated with infective endocarditis.

The Journal of thoracic and cardiovascular surgery·1995
Same author

[Various immunoproliferative disorders].

Nihon Naika Gakkai zasshi. The Journal of the Japanese Society of Internal Medicine·1995

Cardiopulmonary bypass (CPB) during heart surgery causes significant leukocyte sequestration in the lungs, leading to leukopenia. Complement system activation and consumption also occur, indicated by C3 fragment generation.

Area of Science:

  • Immunology
  • Cardiovascular Surgery
  • Critical Care Medicine

Background:

  • Cardiopulmonary bypass (CPB) is a critical procedure in open-heart surgery.
  • The physiological effects of CPB on the immune system, particularly complement activation and leukocyte behavior, are not fully understood.

Purpose of the Study:

  • To investigate complement system activation and pulmonary leukocyte sequestration during CPB in patients undergoing open-heart surgery.
  • To analyze the changes in complement component levels and identify alterations in C3 molecule fragments.

Main Methods:

  • Monitoring of complement components (C3, C4, CH50) and leukocyte counts in 20 patients during CPB.
  • Utilizing immunoblotting to detect alterations and fragments of the C3 molecule in patient plasma.

Related Experiment Videos

Main Results:

  • All patients exhibited pulmonary leukocyte sequestration and complement conversion during CPB.
  • Systemic neutropenia was observed early in CPB, followed by neutrocytosis, with transpulmonary sequestration contributing significantly to leukopenia.
  • Decreased plasma C3, C4, and CH50 levels were noted, alongside the appearance of C3a and C3b fragments, suggesting complement activation.
  • A novel C3 fragment (14,000 MW) was detected, indicating complement component consumption independent of activation.

Conclusions:

  • CPB triggers significant pulmonary leukocyte sequestration and complement system alterations.
  • Both complement activation and non-specific complement consumption occur during CPB.
  • These findings highlight the complex immune response during CPB and its potential clinical implications.