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Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay
Published on: March 1, 2011
Identification of interleukin-1 in normal human lymph derived from skin
J Plachta1, W L Olszewski, I Grzelak
1Department of Surgical Research & Transplantology, Polish Academy of Sciences, Warsaw.
Lymphokine Research
|January 1, 1988
Abstract:
Interleukin 1 was isolated from human lymph drained from normal skin. The lymph was separated by gel filtration chromatography on Spherogel TSK SW 2000 column. IL-1 activity was found in three fractions: greater than 70 kD, 13-16 kD and 5-5.5 kD. Additionally a slight IL-1 activity was found in the 36-40 kD fraction. When exogenous IL-1 was added to the lymph inhibitory activity was detected in fractions: greater than 70 kD, 22-30 kD and 5.5-8 kD.
